circrnas chip arraystar human circrnas chip  (Arraystar inc)

Journal: Communications Biology

Article Title: Circular RNA cVIM promotes hepatic stellate cell activation in liver fibrosis via miR-122-5p/miR-9-5p-mediated TGF-β signaling cascade

doi: 10.1038/s42003-024-05797-3

Figure Lengend Snippet: Masson staining was performed in CCl 4 mice ( a ) and BDL mice ( b ), n = 6 mice per group. Scale bar, 100 μm. c Heat map for differentially expressed circRNAs analyzed by circRNA arraystar ChIP between the fibrotic tissues and the control tissues ( n = 3 per group). Expression of cVIM in the fibrotic tissues in CCl 4 ( d ) and BDL mice ( e ), n = 6 mice per group. f Expression of cVIM in primary HSCs isolated from healthy mice during culture days ( n = 6 per group). Each value is the mean ± SD of six independent experiments. ** P < 0.01 compared to the control.

Article Snippet: Scale bar, 100 μm. c Heat map for differentially expressed circRNAs analyzed by circRNA arraystar ChIP between the fibrotic tissues and the control tissues ( n = 3 per group).

Techniques: Staining, Control, Expressing, Isolation

Journal: Molecular Therapy. Nucleic Acids

Article Title: The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms

doi: 10.1016/j.omtn.2023.08.017

Figure Lengend Snippet: Circular RNAs are deregulated in human abdominal aortic aneurysm (A) Volcano plot depicting downregulated (51, blue) and upregulated (40, red). Circular RNAs (circRNAs) in human elective human abdominal aortic aneurysm (eAAA, n = 11) vs . control (CTRL, n = 6) aorta specimens, as resulted by array experiments. Log2 fold change and -log10 p value are plotted on the x and y axes, respectively. IDs of circRNAs meant for a first round of validation are highlighted. Statistics: unpaired t test; p values <0.05 are considered significant. (B) Pie chart illustrating the proportion of exonic (89.8%), intronic (5.7%), sense overlapping (3.4%), and antisense (1.1%) array-identified differentially expressed circRNAs. Absolute numbers are further indicated for each group. (C) Real-time quantitative PCR (qPCR) validation of hsa_circ_0005660 (c NFIX ), hsa_circ_0003641 (c ATM ), hsa_circ0042103 (c MYOCD ), hsa_circ003218 (c BMPR2 ), hsa_circ0004771 (c NRIP1 ), and hsa_circ0005615 (c NFATC3 ) differential expression in human eAAA (N = 8) and CTRL (N = 4) aortas. 2 –ddCT was calculated by normalizing on RPLPO . Data are represented as mean ± SEM. Statistics: unpaired t test; p values <0.05 are considered significant. NS, not significant; eAAA, elective AAA.

Article Snippet: The resulting labeled cDNA was then purified and 1 μg was fragmented, heated, and subsequently hybridized with an 8 × 15k commercially available array chip displaying 13,617 human circRNAs (Arraystar, no. AS-S-CR-H-V2.0) for 17 h at 65°C in an Agilent Hybridization Oven.

Techniques: Control, Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative Proteomics

Journal: Cell Death Discovery

Article Title: CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression

doi: 10.1038/s41420-021-00706-7

Figure Lengend Snippet: A The heat map shows the 20 differently expressed circRNAs, which were analyzed by circRNAs Arraystar Chip. B The relative mRNA expression of 10 down-regulated circRNAs were detected by qRT-PCR ( n = 10/group). C The relative mRNA expression of 10 up-regulated circRNAs were detected by qRT-PCR ( n = 10/group). D Scheme illustrating the production of circ-NNT. Circ-NNT was formed by back splicing at exon 17 of the NNT gene. E The relative mRNA expression of circ-NNT was shown in a scatter plots (the top upregulated circRNAs), which was measured by qRT-PCR ( n = 10/group). F The gel electrophoresis validated the existence of circ-NNT. G Circ-NNT and NNT mRNA levels in cardiomyocytes with or without RNase R treatment were measured by qRT-PCR. ns: no significant, *** P < 0.0001.

Article Snippet: A The heat map shows the 20 differently expressed circRNAs, which were analyzed by circRNAs Arraystar Chip.

Techniques: Expressing, Quantitative RT-PCR, Nucleic Acid Electrophoresis